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GeticoFect CHO Transfection Kit Instruction Manual1. IntroductionThe GeticoFect CHO Transfection Kit, developed by Geneticsci Biotech (Shanghai) Co., Ltd., is a state - of - the -
GeticoFect CHO Transfection Kit Instruction Manual1. IntroductionThe GeticoFect CHO Transfection Kit, developed by Geneticsci Biotech (Shanghai) Co., Ltd., is a state - of - the -
Catalog Number | Specification | Price (CNY) |
181301 | For 1L | 9,800.00 |
181302 | For 10L | 88,200.00 |
181303 | For 50L | 396,000.00 |
In the fields of biopharmaceuticals and biotechnology research, Chinese Hamster Ovary (CHO) cells have become the preferred host cells for producing therapeutic proteins, antibodies, and other biological products. This is attributed to their excellent protein expression capability, favorable growth characteristics, and glycosylation modification patterns similar to those of human proteins. Achieving efficient transient transfection is crucial for rapidly obtaining large quantities of target proteins. GeticoFect CHO Transfection Reagent is a professional product meticulously designed to meet this demand. Specifically tailored for the transient transfection of CHO cells in high-density culture, it delivers outstanding performance both in small-scale laboratory research and large-scale industrial production.
GeticoFect CHO Transfection Reagent is more than just a single reagent; it is complemented by optimized transfection enhancers and culture additives, forming a powerful transfection solution. This high-efficiency proprietary transfection reagent acts like a precise key, unlocking the door to high-efficiency protein expression in CHO cells. When CHO cells are cultured in CHO expression medium, the synergistic effect of this reagent and its supporting products enables extremely high protein expression levels and yields. This means researchers and production personnel can obtain more target proteins in a shorter time and at a lower cost, providing strong support for the research, development, and production of biological products.
By using matching transfection enhancers and culture feeds, the transfection performance and protein expression can be significantly improved. In high-density suspension CHO cell cultures, this kit can achieve a transfection efficiency of over 90%. Imagine that in a culture system, most CHO cells can successfully take up exogenous genes and express them, which will greatly increase the yield of target proteins. This high transfection efficiency benefits from the optimization of the transfection process by the transfection enhancer—it can promote the binding, internalization of exogenous genes with cells, and their expression in cells. Meanwhile, the culture feed provides sufficient nutrients and a suitable growth environment for cells, ensuring that cells maintain a good state and activity during the transfection process.
Only about half the amount of plasmid DNA required by other transfection reagents is needed to achieve the same or better expression efficiency. In genetic engineering experiments, the preparation and purification of plasmid DNA is a cumbersome and costly process. However, GeticoFect CHO Transfection Kit can achieve or even surpass the effects of other reagents with less plasmid DNA. This not only saves experimental costs but also reduces the workload and time consumption in the plasmid preparation process. This advantage allows researchers to conduct experiments more efficiently and also lowers the cost input in industrial production.
The kit provides a variety of transient expression protocols to maximize work flexibility. Different research and production needs may require different expression conditions and strategies, and GeticoFect CHO Transfection Kit fully takes this into account, providing users with multiple options. Whether adjusting transfection time, culture conditions, or other parameters, flexible settings can be made according to specific situations. At the same time, it is still applicable to the same overall workflow used by other transient expression systems. This means users can easily apply the kit to existing work without re-establishing complex experimental systems, greatly improving work efficiency and compatibility.
It can achieve transfection results with good stability and reproducibility, giving customers more confidence in the results. In scientific research and production, the stability and reproducibility of experimental results are very important. If the results of each experiment vary greatly, reliable conclusions cannot be drawn, and the quality and consistency of products cannot be guaranteed. GeticoFect CHO Transfection Kit has undergone strict quality control and optimization, and can maintain stable transfection efficiency and protein expression levels under different experimental conditions and batches. This allows researchers and production personnel to use the kit with greater confidence, reducing experimental errors and uncertainties, and improving the reliability of research and production.
The culture volume can be adjusted arbitrarily. Whether for small-scale laboratory research or large-scale industrial production, GeticoFect CHO Transfection Kit can adapt to different culture volume requirements. This flexibility makes the kit have a wider range of applications. Whether conducting preliminary exploratory research in the laboratories of scientific research institutions or large-scale protein production in the production workshops of biopharmaceutical enterprises, the appropriate culture volume can be selected according to the actual situation to give full play to the advantages of the kit.
The transfection kit includes cationic GeticoFect CHO Transfection Reagent, GeticoFect CHO Transfection Enhancer, and ExCHO Cell Culture Additive. The cationic GeticoFect CHO Transfection Reagent can combine with negatively charged exogenous genes to form stable complexes, and then introduce the exogenous genes into CHO cells through interaction with the cell membrane. GeticoFect CHO Transfection Enhancer further optimizes the transfection process, improving transfection efficiency and protein expression levels. ExCHO Cell Culture Additive provides rich nutrients and growth factors for CHO cells, maintaining a good growth state of cells and promoting cell proliferation and protein expression. These three components cooperate with each other to form a complete transfection system, providing comprehensive support for the efficient transient transfection of CHO cells.
In conclusion, GeticoFect CHO Transfection Reagent and its supporting transfection kit, with their efficient transfection performance, significant advantages, and rich composition, provide an ideal solution for the transient transfection of CHO cells. Whether in the field of scientific research or industrial production, it will become a powerful assistant for researchers and production personnel, promoting the continuous development of biopharmaceuticals and biotechnology.
Group | Protein Output (mg/L) |
Gibco | 1290.6 |
Getico | 2950.2 |
Group | Cell Amount (Day 1) | Cell Amount (Day 5) | Cell Amount (Day 9) | Cell Amount (Day 14) |
Gibco | 1.83 | 1.50 | 1.34 | 0.20 |
Getico | 2.10 | 2.39 | 1.96 | 0.16 |
From the comparison results, it can be seen that compared with Thermo Gibco's kit, ours achieves better results in protein expression yield and cell activity, with an overall effect exceeding 50%-100%. This can effectively reduce the cost of drug production.
Q: What are the core components of GeticoFect™ CHO Transfection Kit?
A: It includes GeticoFect™ CHO Reagent, GeticoFect™ CHO Enhancer. When combined with supporting ExpiCHO Expression Medium and feeds, it forms a complete solution, eliminating the need for additional procurement of other key consumables.
Q: What scenarios is this kit suitable for?
A: It is mainly used for transient transfection of CHO cells (such as ExpiCHO-S cells) in high-density suspension culture. It widely supports the research, development, and production of biological products such as antibodies and protein drugs. It can also be applied to scenarios such as gene therapy vectors (e.g., virus-like particles for HIV vaccines) and functional expression of complex membrane proteins.
Q: What are the storage requirements?
A: It should be stored at 2–8°C in the dark. The reagent can be rapidly complexed (1–5 minutes) directly at 4°C without the need for pre-warming, simplifying the operation process.
Q: What is the transfection efficiency and protein yield?
A: The transfection efficiency is as high as over 90%, and it supports multiple expression protocols:
• Standard protocol: Antibody yield 1.5–2.0 g/L, culture duration 8–10 days, cell viability > 85%;
• High-titer protocol: Antibody yield 2.5–3.0 g/L, culture duration 10–12 days, cell viability > 75%;
• Maximum-titer protocol: Antibody yield > 3.0 g/L, culture duration 12–14 days, cell viability > 70%.
Q: What advantages does it have compared with traditional transfection methods (such as PEI, electroporation)?
A: Compared with PEI (transfection efficiency 30–50%, yield 0.1–0.5 g/L) and electroporation (transfection efficiency 50–70%, yield 0.5–1.0 g/L), GeticoFect™ CHO has higher transfection efficiency (90%+), significantly improved yield (3.0 g/L+), low cytotoxicity (viability > 90%), simpler operation (three-step method, no professional equipment required), and supports seamless scaling from 125 mL shake flasks to 2000 L bioreactors.
Q: Why is it said to be more effective than Thermo's CHO kit?
A: In large-scale production tests, the overall effect and protein yield of GeticoFect™ CHO Kit are more than 50%-100% higher than those of Thermo (Gibco) Kit. For example, within the same culture cycle, the protein output of GeticoFect (295.2 mg/L) is significantly higher than that of Gibco (129.6 mg/L), and the cell activity is maintained more stably (on the 9th day of culture, the cell amount of GeticoFect is 1.96, while that of Gibco is 1.34).
Q: Is the operation process complicated?
A: The operation is simple, no medium change is required, and transfection can be completed directly in ExpiCHO Medium:
• Day -1: Inoculate cells to a density of 3-4×10⁶ cells/mL and culture overnight;
• Day 0: Dilute cells to 6×10⁶ cells/mL, prepare DNA-reagent complexes (complexation for 1–5 minutes), add to cells, and incubate with shaking at 37°C;
• Add enhancers and feeds according to the protocol in the subsequent steps, and harvest the supernatant after 8–14 days.
Q: Can it support large-scale production?
A: It supports seamless scaling from 125 mL shake flasks to 50 L bioreactors without adjusting the core process, making it suitable for the entire workflow from laboratory research and development to industrial production.
Q: What advantages does it have compared with the HEK293 expression system?
A: The yield is increased by an average of 2.4 times, and the protein glycosylation pattern is closer to the natural state. The antibody-dependent cellular cytotoxicity (ADCC) activity is higher, making it more suitable for the production of protein drugs requiring high biological activity.
Q: What are the requirements for cell status?
A: It is recommended that the cell density during transfection is 6×10⁶ cells/mL, and the cells must be in the logarithmic growth phase to ensure transfection efficiency and yield.
Q: Is it necessary to optimize the DNA concentration?
A: It is recommended that the DNA concentration is 0.5–1.0 μg/mL of the culture volume (0.8 μg/mL is recommended), and fine-tuning can be performed according to the specific protein type to obtain the best effect.
Q: Does temperature have an impact on expression?
A: Culturing at 32°C can extend the expression cycle and increase the yield. The high-titer and maximum-titer protocols recommend using the culture condition of 32°C.
