GeticoFect mRNA Transfection Reagent Introduction
Product Ordering Information
Catalog Number | Specification | Price (CNY) |
130301 | 0.75ml | 3,000.00 |
130302 | 1.5ml | 4,850.00 |
130303 | 15ml | 43,500.00 |
1. Product Overview
GeticoFect mRNA Transfection Reagent, developed by Getico Scientific (Shanghai) Co., Ltd., is a state-of-the-art transfection solution meticulously engineered for the highly efficient delivery of messenger RNA (mRNA) into a wide spectrum of eukaryotic cells. This reagent represents a significant advancement in the field of gene delivery, offering researchers a powerful tool to manipulate gene expression and study cellular functions with mRNA. It is designed to meet the diverse needs of modern molecular biology research, from basic studies on gene function to the development of mRNA-based therapeutics.
2. Key Features
2.1 Exceptionally High Transfection Efficiency
- Advanced Formulation for Maximum mRNA Uptake: GeticoFect mRNA Transfection Reagent features a cutting-edge formulation that enables the formation of highly stable and fusogenic complexes with mRNA. These complexes are optimized to efficiently fuse with the cell membrane, ensuring a high rate of mRNA uptake by the target cells. In common cell lines such as HEK-293 and HeLa, transfection efficiencies of up to 90% have been consistently achieved. For example, when transfecting a reporter mRNA encoding a fluorescent protein into HEK-293 cells, over 85% of the cells exhibit strong fluorescence 24 hours post-transfection, indicating successful mRNA delivery and translation. This high efficiency is also maintained in more challenging cell types, such as primary neurons and certain stem cells, where it outperforms many traditional transfection reagents.
- Optimized for Different mRNA Sizes and Structures: Whether you are working with short, synthetic mRNAs for gene knockdown experiments or long, full-length mRNAs for protein overexpression studies, GeticoFect mRNA Transfection Reagent demonstrates remarkable versatility. It can effectively deliver mRNAs of various sizes and structures, ensuring reliable results across different experimental applications. This ability to handle diverse mRNA molecules makes it an invaluable tool for precise gene manipulation in both basic research and translational applications.
2.2 Ultra-Low Cytotoxicity
- Gentle on Cells for Sustained Health: The reagent is engineered to be minimally cytotoxic to cells. After transfection, cell viability typically remains above 95% in most cell lines. This is crucial for maintaining the physiological integrity of the cells during long-term experiments, such as those involving the study of gene function over several days or weeks. In contrast to some traditional transfection reagents that can cause significant cell death and non-specific cellular stress, GeticoFect mRNA Transfection Reagent ensures that cells remain healthy and functional, providing more reliable and physiologically relevant results. For example, in a 7-day experiment to study the effect of mRNA transfection on cell metabolism, cells transfected with GeticoFect mRNA Transfection Reagent maintain their normal metabolic profiles, while cells transfected with a more cytotoxic reagent show signs of metabolic disruption.
2.3 Broad Cell-Type Compatibility
- Versatile Application Across Cell Types: GeticoFect mRNA Transfection Reagent is compatible with an extensive range of cell types, including common cell lines, primary cells, and stem cells. It has been successfully applied in over 70 different cell lines, covering cancer cell lines like MCF-7 and A549, as well as primary fibroblasts, endothelial cells, and various types of stem cells (embryonic stem cells, induced pluripotent stem cells, and adult stem cells). In stem cell research, it has enabled efficient transfection of mRNAs involved in stem cell differentiation, self-renewal, and lineage commitment. This broad cell-type compatibility makes it a versatile choice for researchers working in diverse fields, from basic cell biology to regenerative medicine and drug development.
2.4 Simplified Transfection Protocol
- User-Friendly and Time-Saving: The transfection protocol for GeticoFect mRNA Transfection Reagent is designed to be simple and straightforward, requiring minimal optimization. It can be used in the presence of serum, eliminating the need for time-consuming and potentially harmful steps such as washing the cells with serum-free media before and after transfection. The reagent also forms complexes with mRNA rapidly, reducing the overall transfection time. For example, in a standard 24-well plate transfection, the entire process from complex formation to addition to the cells can be completed within 30 minutes, compared to some other reagents that may take several hours. This simplicity and efficiency make it suitable for both novice researchers and those conducting high-throughput experiments.
3. Applications
3.1 Gene Expression Studies
- Precise Manipulation of Protein Expression: In basic research, GeticoFect mRNA Transfection Reagent is an essential tool for studying gene function. By efficiently delivering mRNA encoding a protein of interest, researchers can rapidly increase the expression of the protein in target cells and observe the resulting phenotypic changes. This approach allows for the investigation of the protein's role in various cellular processes, such as cell cycle regulation, signal transduction, and metabolism. For example, in a study on the role of a particular protein in cell migration, transfection of mRNA encoding the protein using GeticoFect mRNA Transfection Reagent led to a significant increase in cell migration, while knockdown of the protein using siRNA resulted in a decrease in cell migration. This precise manipulation of protein expression levels enables researchers to establish clear cause-and-effect relationships between genes and cellular functions.
3.2 Therapeutic Target Validation
- Accelerating Drug Development: In the pharmaceutical industry, GeticoFect mRNA Transfection Reagent plays a crucial role in validating potential therapeutic targets. By delivering mRNA encoding a therapeutic protein or a gene-editing tool (such as CRISPR-Cas9 mRNA) into cells, researchers can test the efficacy of the treatment in a cellular model. For example, in cancer research, mRNA encoding a tumor suppressor protein can be transfected into cancer cells using GeticoFect mRNA Transfection Reagent to assess its ability to inhibit tumor growth. If the transfection leads to a reduction in cancer cell viability or an inhibition of tumor-related phenotypes, it provides strong evidence that targeting this gene could be a promising therapeutic approach. This helps pharmaceutical companies prioritize their drug-development efforts and increases the likelihood of developing effective drugs.
3.3 Vaccine Development
- Enabling mRNA Vaccine Research: GeticoFect mRNA Transfection Reagent is also well-suited for vaccine development. mRNA vaccines have emerged as a promising approach for preventing and treating infectious diseases and cancers. The reagent can be used to deliver mRNA encoding antigens into antigen-presenting cells (such as dendritic cells), which then process and present the antigens to the immune system, triggering an immune response. In preclinical studies, GeticoFect mRNA Transfection Reagent has been used to efficiently transfect dendritic cells with mRNA encoding viral antigens, resulting in the production of high levels of antigen-specific T cells and antibodies. This technology holds great potential for the development of safe and effective mRNA vaccines.
4. Product Packaging and Storage
- Convenient Packaging: GeticoFect mRNA Transfection Reagent is available in a convenient 1 ml vial, which is sufficient for a large number of transfection experiments. The vial is designed for easy handling and dispensing, ensuring minimal reagent waste. For example, in 24-well plate transfections, one vial can support approximately 300 - 600 transfections, depending on the specific experimental conditions.
- Storage Conditions: Store the reagent at 4°C. Do not freeze. When stored under the recommended conditions, the reagent has a shelf life of 12 months from the date of manufacture. Avoid repeated exposure to room temperature, as this may affect the reagent's performance. Return the reagent to 4°C immediately after use.
In conclusion, GeticoFect mRNA Transfection Reagent is a powerful, versatile, and user-friendly tool for mRNA delivery in eukaryotic cells. Its unique combination of high transfection efficiency, ultra-low cytotoxicity, broad cell-type compatibility, and simplified protocol makes it an ideal choice for a wide range of research applications, from basic science to drug development and vaccine research.
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