GeticoFect HQ Transfection Reagent Introduction
Product Ordering Information
Catalog Number | Specification | Price (CNY) |
130801 | 0.75ml | 2,880.00 |
130802 | 1.5ml | 4,650.00 |
130803 | 15ml | 41,850.00 |
1. Product Overview
GeticoFect HQ Transfection Reagent, developed by Getico Scientific (Shanghai) Co., Ltd., is a high - performance transfection solution designed to revolutionize the delivery of nucleic acids into eukaryotic cells. Engineered with advanced technology, this reagent is a go - to choice for researchers across various fields, from basic cell biology to pharmaceutical research. It is optimized to handle a wide array of nucleic acid types, including DNA (plasmids), RNA, siRNA, and miRNA, catering to diverse experimental needs.
2. Key Features
2.1 Exceptionally High Transfection Efficiency
- Advanced Formulation for Maximum Uptake: GeticoFect HQ features a cutting - edge formulation that enables the formation of highly stable and fusogenic complexes with nucleic acids. These complexes are designed to efficiently fuse with the cell membrane, ensuring a high rate of nucleic acid uptake by the target cells. In common cell lines such as HEK - 293 and CHO , transfection efficiencies of up to 95% have been consistently achieved. For instance, when transfecting a plasmid DNA encoding a luciferase reporter gene into HEK - 293 cells, over 90% of the cells exhibit strong luciferase activity 48 hours post - transfection, indicating successful gene delivery and expression. This high efficiency is also maintained in more challenging cell types, such as primary neurons and certain stem cells, where it outperforms many traditional transfection reagents.
- Optimized for Different Nucleic Acid Sizes: Whether you are working with large - sized plasmids for gene over - expression studies or small siRNA molecules for gene knockdown experiments, GeticoFect HQ demonstrates remarkable versatility. It can efficiently deliver siRNA to achieve gene silencing efficiencies of up to 85% in a wide range of cell types. This ability to handle nucleic acids of varying sizes makes it an invaluable tool for precise gene manipulation in both basic research and translational applications.
2.2 Ultra - Low Cytotoxicity
- Gentle on Cells for Sustained Health: The reagent is engineered to be minimally cytotoxic to cells. After transfection, cell viability typically remains above 98% in most cell lines. This is crucial for maintaining the physiological integrity of the cells during long - term experiments, such as those involving the study of gene function over several days or weeks. In contrast to some traditional transfection reagents that can cause significant cell death and non - specific cellular stress, GeticoFect HQ ensures that cells remain healthy and functional, providing more reliable and physiologically relevant results. For example, in a 10 - day experiment to study the effect of gene knockdown on cell metabolism, cells transfected with GeticoFect HQ maintain their normal metabolic profiles, while cells transfected with a more cytotoxic reagent show signs of metabolic disruption.
2.3 Broad Cell - Type Compatibility
- Versatile Application Across Cell Types: GeticoFect HQ is compatible with an extensive range of cell types, including common cell lines, primary cells, and stem cells. It has been successfully applied in over 80 different cell lines, covering cancer cell lines like MCF - 7 and A549, as well as primary fibroblasts, endothelial cells, and various types of stem cells (embryonic stem cells, induced pluripotent stem cells, and adult stem cells). In stem cell research, it has enabled efficient transfection of genes involved in stem cell differentiation, self - renewal, and lineage commitment. This broad cell - type compatibility makes it a versatile choice for researchers working in diverse fields, from basic cell biology to regenerative medicine and drug development.
2.4 Simplified Transfection Protocol
- User - Friendly and Time - Saving: The transfection protocol for GeticoFect HQ is designed to be simple and straightforward, requiring minimal optimization. It can be used in the presence of serum, eliminating the need for time - consuming and potentially harmful steps such as washing the cells with serum - free media before and after transfection. The reagent also forms complexes with nucleic acids rapidly, reducing the overall transfection time. For example, in a standard 24 - well plate transfection, the entire process from complex formation to addition to the cells can be completed within 30 minutes, compared to some other reagents that may take several hours. This simplicity and efficiency make it suitable for both novice researchers and those conducting high - throughput experiments.
3. Applications
3.1 Gene Expression Studies
- Precise Manipulation of Gene Expression: In basic research, GeticoFect HQ is an essential tool for studying gene function. By efficiently delivering plasmid DNA encoding a gene of interest, researchers can over - express the gene in target cells and observe the resulting phenotypic changes. Conversely, when used to deliver siRNA or miRNA, it can knockdown the expression of specific genes, allowing for the investigation of the gene's role in cellular processes. For example, in a study on the role of a particular gene in cell - cycle regulation, transfection of plasmid DNA encoding the gene using GeticoFect HQ led to a significant increase in cell proliferation, while transfection of siRNA targeting the same gene resulted in cell - cycle arrest. This precise manipulation of gene expression levels enables researchers to establish clear cause - and - effect relationships between genes and cellular functions.
3.2 Therapeutic Target Validation
- Accelerating Drug Development: In the pharmaceutical industry, GeticoFect HQ plays a crucial role in validating potential therapeutic targets. By knocking down genes that are implicated in disease processes, researchers can determine whether inhibiting these genes could be a viable strategy for drug development. For example, in cancer research, siRNA delivered by GeticoFect HQ has been used to target genes associated with tumor growth, invasion, and metastasis. If gene knockdown leads to a reduction in cancer cell viability or an inhibition of tumor - related phenotypes, it provides strong evidence that targeting this gene could be a promising therapeutic approach. This helps pharmaceutical companies prioritize their drug - development efforts and increases the likelihood of developing effective drugs.
3.3 High - Throughput Screening
- Efficient Screening for Functional Genomics: GeticoFect HQ is well - suited for high - throughput screening applications. Its high transfection efficiency, low cytotoxicity, and simplified protocol allow for the rapid and reliable transfection of large numbers of nucleic acid libraries. In a large - scale screen to identify genes involved in a specific cellular process, such as cell - cycle regulation or immune response, thousands of different siRNA or plasmid DNA constructs can be transfected into cells using GeticoFect HQ. The high transfection efficiency ensures that each well in a multi - well plate contains a sufficient number of transfected cells for accurate phenotypic analysis. This enables researchers to quickly identify genes that are essential for the process of interest, accelerating the pace of discovery in functional genomics research.
4. Product Packaging and Storage
- Convenient Packaging: GeticoFect HQ Transfection Reagent is available in a convenient 1 ml vial, which is sufficient for a large number of transfection experiments. The vial is designed for easy handling and dispensing, ensuring minimal reagent waste. For example, in 24 - well plate transfections, one vial can support approximately 400 - 800 transfections, depending on the specific experimental conditions.
- Storage Conditions: Store the reagent at 4°C. Do not freeze. When stored under the recommended conditions, the reagent has a shelf life of 12 months from the date of manufacture. Avoid repeated exposure to room temperature, as this may affect the reagent's performance. Return the reagent to 4°C immediately after use.
In conclusion, GeticoFect HQ Transfection Reagent is a powerful, versatile, and user - friendly tool for nucleic acid delivery in eukaryotic cells. Its unique combination of high transfection efficiency, ultra - low cytotoxicity, broad cell - type compatibility, and simplified protocol makes it an ideal choice for a wide range of research applications, from basic science to drug development.
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