GeticoFect RNAiPlus Transfection Reagent: Unparalleled Precision in RNAi Delivery
Product Ordering Information
Catalog Number | Specification | Price (CNY) |
133201 | 0.75ml | 3,800.00 |
133202 | 1.5ml | 6,200.00 |
133203 | 15ml | 53,800.00 |
1. Product Overview
GeticoFect RNAiPlus Transfection Reagent, innovatively developed by Getico Scientific (Shanghai) Co., Ltd., represents a quantum leap in the realm of RNA interference (RNAi) - mediated gene silencing. Tailored to meet the intricate demands of modern molecular research, this reagent is designed for the highly efficient delivery of small interfering RNA (siRNA) and microRNA (miRNA) into a diverse array of eukaryotic cells. It serves as an indispensable tool for researchers aiming to precisely manipulate gene expression at the post - transcriptional level, unlocking new insights into biological processes and disease mechanisms.
2. Key Features
2.1 Unrivaled Transfection Efficiency
- Advanced Formulation for siRNA Delivery: GeticoFect RNAiPlus has been meticulously engineered with a cutting - edge formulation that enables the encapsulation and delivery of siRNA molecules with unprecedented efficiency. In a wide range of cell lines, it consistently achieves transfection efficiencies exceeding 95%, even in the most recalcitrant cell types. For example, in the notoriously difficult - to - transfect PC - 3 prostate cancer cells, GeticoFect RNAiPlus has demonstrated transfection efficiencies as high as 96%, far surpassing many conventional transfection reagents. This high efficiency ensures that a vast majority of cells internalize the siRNA, leading to robust gene knockdown and minimizing background expression from untransfected cells.
- Minimal siRNA Requirements: It can achieve profound gene knockdown with extremely low concentrations of siRNA. In numerous experiments, as little as 0.5 nM of siRNA has been sufficient to achieve high - level gene silencing. This not only significantly reduces the cost of siRNA synthesis but also minimizes potential off - target effects associated with higher siRNA concentrations. By using such low siRNA amounts, researchers can precisely target their genes of interest and obtain highly accurate results in their RNAi experiments.
2.2 Ultra - Low Cytotoxicity for Sustained Cell Health
- Gentle on Cellular Physiology: The reagent is formulated to be exquisitely gentle on cells, resulting in ultra - low cytotoxicity. After transfection with GeticoFect RNAiPlus, cell viability remains above 98% across a broad spectrum of cell lines. This stands in sharp contrast to some traditional transfection reagents that can cause substantial cell death and non - specific cellular stress. In long - term RNAi experiments, such as those investigating the effects of gene knockdown over several days or weeks, the low cytotoxicity of GeticoFect RNAiPlus ensures that cell functions remain uncompromised, allowing for more reliable and physiologically relevant results. For instance, in cell - based assays that require continuous monitoring of cell growth, differentiation, and behavior after gene knockdown, the high cell viability maintained by this reagent enables accurate assessment of the true effects of gene silencing.
2.3 Broad - Spectrum Cell - Type Compatibility
- Versatility in Application: GeticoFect RNAiPlus is compatible with an extensive range of cell types, including common cell lines, stem cells, and primary cells. It has been successfully employed in over 80 different cell lines, spanning from cancer cell lines like MDA - MB - 231 and HT - 29 to primary neurons and endothelial cells. In stem cell research, it has efficiently transfected hematopoietic stem cells and neural stem cells, facilitating in - depth studies on gene function during stem cell differentiation, self - renewal, and lineage commitment. This broad cell - type compatibility makes it an ideal choice for researchers working in diverse fields, from basic cell biology to regenerative medicine and translational research.
2.4 Enhanced miRNA Transfection Capabilities
- Superior miRNA Delivery: In addition to its remarkable performance in siRNA transfection, GeticoFect RNAiPlus also excels in delivering miRNA mimics and antagonists. It can efficiently introduce these miRNA - based molecules into cells, enabling researchers to explore the complex miRNA - mediated gene regulation pathways with greater precision. For example, in experiments aimed at elucidating the role of a specific miRNA in cell proliferation, apoptosis, or metastasis, GeticoFect RNAiPlus has achieved high transfection efficiencies, leading to significant and reproducible changes in the target gene expression levels associated with the miRNA of interest.
3. Applications
3.1 In - Depth Gene Function Studies
- Precision Gene Knockdown: In basic research, GeticoFect RNAiPlus is an invaluable asset for studying gene function. By efficiently delivering siRNA to knockdown specific genes, researchers can meticulously observe the resulting phenotypic changes in cells. For example, in a study on the role of a particular gene in cell - cell adhesion, transfection with GeticoFect RNAiPlus - delivered siRNA targeting that gene led to a significant disruption in cell - cell contacts, as measured by immunofluorescence and cell - aggregation assays. This precise gene - knockdown capability allows researchers to establish clear cause - and - effect relationships between genes and cellular functions, providing crucial insights into normal biological processes and the pathogenesis of diseases.
- Elucidating Gene - Regulatory Pathways: It is also instrumental in identifying gene - regulatory pathways. Through systematic knockdown of genes within a suspected pathway using GeticoFect RNAiPlus, researchers can map out the intricate relationships between different genes and understand how they interact to regulate cellular processes. In cancer research, for instance, this approach has been used to identify key genes in the Wnt - β - catenin pathway, which is frequently dysregulated in many types of cancers. By knocking down individual genes in this pathway using siRNA delivered by GeticoFect RNAiPlus, researchers were able to decipher the sequence of events and the specific roles of each gene in promoting cancer cell growth, invasion, and metastasis.
3.2 Therapeutic Target Validation and Drug Discovery
- Accelerating Drug Development: In the pharmaceutical industry, GeticoFect RNAiPlus plays a pivotal role in validating potential therapeutic targets. By knocking down genes that are implicated in disease processes, researchers can determine whether inhibiting these genes could be a viable strategy for drug development. For example, in neurodegenerative disease research, siRNA delivered by GeticoFect RNAiPlus has been used to target genes associated with abnormal protein aggregation, such as the huntingtin gene in Huntington's disease. If gene knockdown leads to a reduction in the formation of toxic protein aggregates and an improvement in cellular function, it provides strong evidence that targeting this gene could be a promising therapeutic approach. This helps pharmaceutical companies prioritize their drug - development efforts and increases the likelihood of developing effective drugs.
- Enabling RNAi - Based Therapeutics: The reagent is also highly relevant for the development of RNAi - based therapeutics. As the field of RNAi therapy continues to advance, the ability to efficiently deliver siRNA or miRNA into target cells in vitro is essential for pre - clinical studies. GeticoFect RNAiPlus's high transfection efficiency and ultra - low cytotoxicity make it an ideal reagent for optimizing the delivery of RNAi agents in cell - based models, which is a critical step towards translating RNAi technology into clinical applications.
3.3 High - Throughput Screening for Rapid Discovery
- Efficient Screening Platform: GeticoFect RNAiPlus is well - equipped for high - throughput siRNA screening. Its streamlined and rapid transfection protocol, combined with its exceptional transfection efficiency, allows for the efficient transfection of large numbers of siRNA libraries in a short period. In a large - scale screen to identify genes involved in a specific cellular process, such as cell cycle regulation or immune response, thousands of different siRNA constructs can be transfected into cells using GeticoFect RNAiPlus. The high transfection efficiency ensures that each well in a multi - well plate contains a sufficient number of transfected cells for accurate phenotypic analysis. This enables researchers to quickly identify genes that are essential for the process of interest, accelerating the pace of discovery in functional genomics research.
4. Comparison with Competitors
5. Product Packaging and Pricing
GeticoFect RNAiPlus Transfection Reagent is available in convenient and user - friendly packaging. A standard vial contains 1 ml of the reagent, which is sufficient for a large number of transfection experiments. The price of GeticoFect RNAiPlus is set at 2000 yuan, offering outstanding value for its high - performance features. Considering its superior transfection efficiency, ultra - low cytotoxicity, broad cell - type compatibility, and enhanced miRNA transfection capabilities, it provides a cost - effective solution for researchers conducting RNAi - related studies. Whether for small - scale academic research projects or large - scale industrial high - throughput screening, GeticoFect RNAiPlus offers a reliable and efficient option without compromising on quality.
In conclusion, GeticoFect RNAiPlus Transfection Reagent is a revolutionary product in the field of RNAi transfection. Its exceptional transfection efficiency, ultra - low cytotoxicity, broad cell - type compatibility, and enhanced miRNA transfection capabilities make it an ideal choice for a wide range of research applications, from basic gene function studies to the development of RNAi - based therapeutics.
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