Language
简体中文 
English GeticoFect 3000Plus Transfection Reagent
GeticoFect 3000Plus Transfection Reagent: A Leap Forward in Transfection Technology1. Product OverviewGeticoFect 3000Plus Transfection Reagent, an innovative offering from Genetics
GeticoFect 3000Plus Transfection Reagent: A Leap Forward in Transfection Technology1. Product OverviewGeticoFect 3000Plus Transfection Reagent, an innovative offering from Genetics
Catalog Number | Specification | Price (CNY) |
131201 | 0.75ml | 2,600.00 |
131202 | 1.5ml | 4,150.00 |
131203 | 15ml | 37,200.00 |
Product Introduction
In the fields of biomedical and biotechnology research, cell transfection technology acts like a bridge connecting basic research and practical applications, playing a pivotal role in key areas such as gene function exploration, drug development, and disease mechanism analysis. An excellent-performing transfection reagent is the core factor determining transfection results and the success or failure of experiments. Developed under such demand, GeticoFect® 3000Plus Transfection Reagent brings a new transfection solution to researchers with its advanced technology and outstanding performance.
GeticoFect® 3000Plus Transfection Reagent innovatively incorporates advanced nanoparticle technology, which, like a shining pearl, endows the reagent with unique and excellent performance. Benefiting from their tiny size and large specific surface area, nanoparticles can form stable and efficient complexes with exogenous nucleic acids (such as DNA, RNA, etc.). These complexes are like precise "transport cabins"—they not only provide comprehensive protection for nucleic acids against degradation by intracellular nucleases but also, relying on their special physicochemical properties, interact subtly with cell membranes. Just like precise navigation, they cross the "barrier" of cell membranes more efficiently and deliver nucleic acids accurately into cells.
In practical applications, the advantages brought by this advanced nanoparticle technology are obvious. Like a skilled "craftsman", it significantly improves the accuracy and reliability of experimental results, making the expression of exogenous genes in cells more stable and efficient. At the same time, due to the precise regulation of nanoparticle technology, the reproducibility of experiments has been greatly enhanced. Researchers can obtain similar and reliable transfection results in different experimental batches, providing a solid guarantee for in-depth scientific research and the verifiability of results.
GeticoFect® 3000Plus Transfection Reagent boasts remarkable transfection efficiency, thanks to the perfect synergy between its unique formula and nanoparticle technology. After forming complexes with exogenous nucleic acids, the reagent, like a sharp "hunter", can quickly recognize and bind tightly to the cell surface, then promote the smooth entry of complexes into cells through various ingenious mechanisms, just like opening "gates" one by one. It shows strong adaptability and efficiency for common cell lines.
Taking HEK293 cells as an example—these are favored cell models in gene expression and protein production research. GeticoFect® 3000Plus Transfection Reagent can quickly and accurately introduce exogenous genes into them for efficient expression, providing abundant experimental data and reliable results for related research. SW480 cells, a type of colorectal cancer cell line, are of great significance in tumor research. The reagent can also efficiently complete the transfection process, helping researchers deeply explore the mechanism of tumor occurrence and development. HepG2 cells are commonly used models for hepatocyte research; the reagent can efficiently deliver exogenous nucleic acids into them, offering strong support for the study of liver diseases and the exploration of drug metabolism. LNCaP cells, a prostate cancer cell line, are crucial for prostate cancer research, and the efficient transfection performance of the reagent opens up new avenues for the research on prostate cancer treatment and diagnosis. P19 cells play an important role in embryonic development and neural differentiation research, and the reagent helps researchers gain a deeper understanding of the mechanism of cell differentiation. As for Hela cells, a classic cell line in tumor research, GeticoFect® 3000Plus Transfection Reagent can also handle them easily, providing strong technical support for the research on tumor-related genes.
Cytotoxicity is one of the key indicators to measure the performance of transfection reagents. During the design and development of GeticoFect® 3000Plus Transfection Reagent, full consideration was given to its impact on cells, just like a careful "guardian". By carefully optimizing the composition and surface properties of nanoparticles, cytotoxicity has been reduced to an extremely low level. During transfection, the interaction between the reagent and cells is gentle and friendly, without significantly interfering with the normal physiological functions of cells. Cells, like in a comfortable "environment", can maintain high activity and vigorous proliferation ability after transfection.
This is particularly important for experiments that require long-term culture and observation. For instance, in some cell signaling pathway studies, it is necessary to observe the signal changes of cells within a period of time after transfection. If the transfection reagent has high cytotoxicity, it may lead to cell death or functional abnormalities, like a "time bomb", seriously affecting the accuracy of experimental results. However, GeticoFect® 3000Plus Transfection Reagent, like a "peacemaker", can avoid these problems and ensure the smooth progress of experiments.
In addition to extremely low cytotoxicity, GeticoFect® 3000Plus Transfection Reagent also has the unique feature of enhancing cell viability. This may be because some components in the reagent, like "nutrients" for cells, can provide certain nutritional support for cells, or act as an "environment regulator" to adjust the intracellular microenvironment and promote cell metabolism and growth. After transfection, cells have a more normal morphology, like energetic "elves", with an accelerated proliferation rate, which helps improve the success rate of experiments and the reliability of data.
For example, in stem cell research, cell viability and self-renewal ability are crucial. After transfection with GeticoFect® 3000Plus Transfection Reagent, stem cells, like being injected with a "source of vitality", maintain good viability and pluripotent differentiation potential, providing a powerful tool for stem cell therapy and regenerative medicine research.
Compared with the company's GeticoFect® 3000 Transfection Reagent, GeticoFect® 3000Plus Transfection Reagent specially adds transfection enhancer T3000. This transfection enhancer, like a "key", effectively increases the transfection efficiency in various hard-to-transfect cells. Hard-to-transfect cells often have unique cell membrane structures, surface charge distributions, or receptor expression patterns, making it difficult for traditional transfection methods to introduce exogenous nucleic acids into them. However, transfection enhancer T3000 can further optimize and modify nanoparticle complexes, enabling them to better interact with the cell membranes of hard-to-transfect cells. Just like "unlocking", it opens the "gates" of cells and promotes the efficient uptake of nucleic acids.
A large number of experimental verifications have shown that when using GeticoFect® 3000Plus Transfection Reagent to transfect hard-to-transfect cells, the transfection efficiency is significantly improved. This provides new possibilities for researchers to study cell types that were previously difficult to tackle, allowing in-depth exploration of more research fields.
Many transfection reagents on the market often "fail miserably" when facing hard-to-transfect cells, with poor results. However, GeticoFect® 3000Plus Transfection Reagent has been deeply studied and optimized specifically for various hard-to-transfect cells. The R&D team, like "scientific detectives", carefully analyzed the characteristics of hard-to-transfect cells, such as cell membrane structure, surface charge, and receptor distribution, and adjusted the formula and surface modification of nanoparticles. As a result, the reagent can interact with these cells better, just like a "customized key".
For example, due to their special physiological state and cell membrane structure, it is difficult for traditional transfection reagents to introduce exogenous nucleic acids into some primary cells. However, through the synergy of optimized nanoparticle technology and transfection enhancer T3000, GeticoFect® 3000Plus Transfection Reagent can effectively overcome these obstacles and achieve efficient transfection of primary cells. This provides a new opportunity for the research of primary cells, helping to gain a deeper understanding of cell physiological functions and disease mechanisms.
Compared with other competitors, GeticoFect® 3000Plus Transfection Reagent has lower cytotoxicity. Like a "gentle partner", it minimizes cell damage while ensuring efficient transfection. Some traditional transfection reagents cause significant damage to cells during transfection, leading to a decrease in cell survival rate and even affecting the normal functions of cells, like a "storm" sweeping through cells. In contrast, relying on its unique nanoparticle design and formula, as well as the synergy of transfection enhancer T3000, GeticoFect® 3000Plus Transfection Reagent behaves gently and friendly during cell transfection.
Moreover, due to its extremely low cytotoxicity and good biocompatibility, there is no need for medium change after transfection. Traditional transfection reagents usually require medium change after transfection to remove the potential impact of residual reagents on cells. However, medium change operation is not only cumbersome, like a "complex project", but also may cause certain damage to cells and increase the risk of experimental contamination. GeticoFect® 3000Plus Transfection Reagent can avoid these problems, greatly simplifying the experimental process, saving time and energy, and improving experimental efficiency. This allows researchers to devote more time and energy to the analysis and research of experimental results.
GeticoFect® 3000Plus Transfection Reagent is like an "all-rounder", suitable for transfection applications of DNA, RNA, and RNAi simultaneously. In gene research, different types of nucleic acids have different functions and application scenarios. DNA is often used in experiments such as gene cloning, expression vector construction, and gene editing, like the "blueprint" of genes; RNA can be used for gene expression regulation and RNA function research, similar to the "regulator" of gene expression; RNAi is an important gene silencing technology used to study gene functions and treat certain diseases, just like the "silencing switch" of genes.
GeticoFect® 3000Plus Transfection Reagent can meet the transfection needs of these different types of nucleic acids, providing researchers with a one-stop transfection solution. Whether it is the transfection of a single nucleic acid or the co-transfection of multiple nucleic acids, the reagent can show excellent performance, like a skilled "all-round performer" that shines on different "stages".
GeticoFect® 3000Plus Reagent exhibits amazing cell compatibility, being compatible with hundreds of cell types. The types of cells it can efficiently transfect are diverse, covering cell lines in multiple fields.
Fibroblasts, such as 3T3 and COS-7 cells, play an important role in tissue repair and regeneration. GeticoFect® 3000Plus Transfection Reagent, like a "messenger", can effectively introduce exogenous genes into these fibroblasts, providing strong support for related research. By transfecting specific genes, the functions and mechanisms of these genes in fibroblasts can be studied, laying a theoretical foundation for the development of tissue engineering and regenerative medicine.
Myoblasts, including C2C12 and L6 CRL-1458 cells, play a key role in muscle development and regeneration. GeticoFect® 3000Plus Transfection Reagent helps researchers efficiently deliver exogenous nucleic acids into myoblasts, promoting their differentiation and proliferation. By transfecting genes related to muscle development, the molecular mechanism of muscle development can be deeply understood, providing new ideas and methods for the treatment and rehabilitation of muscle diseases.
Hepatocytes, such as HepG2 and HuH7 cells, play important roles in substance metabolism, detoxification, and immunity. GeticoFect® 3000Plus Transfection Reagent provides an efficient transfection tool for gene research of hepatocytes. By transfecting genes related to liver diseases, the pathogenesis of liver diseases can be studied, and drugs for treating liver diseases can be screened, providing a theoretical basis for the prevention and treatment of liver diseases.
Erythroleukemia cells, such as K562 cells, are of great significance in the research on the pathogenesis and treatment of leukemia. GeticoFect® 3000Plus Transfection Reagent can meet the transfection needs of erythroleukemia cells. By transfecting genes related to leukemia, the pathogenesis of leukemia can be deeply understood, and new treatment strategies can be developed.
Lung cancer cells, such as A549 and NCI-H460 cells, are important models for lung cancer research. GeticoFect® 3000Plus Transfection Reagent provides reliable support for gene research of lung cancer cells. By transfecting genes related to the occurrence and development of lung cancer, the pathogenesis of lung cancer can be studied, and diagnostic markers and therapeutic targets for lung cancer can be screened, providing a theoretical basis for the early diagnosis and personalized treatment of lung cancer.
The operation process of GeticoFect® 3000Plus Transfection Reagent is simple and easy to understand, like a clear "instruction manual". Researchers can complete the transfection process by following the specified steps. No complex technology or tedious preparation work is required, which reduces the difficulty and threshold of experiments and allows more researchers to get started easily. This not only improves experimental efficiency but also reduces experimental errors and failures caused by improper operation, providing convenience for the smooth progress of scientific research.
For common cell types, compared with other reagents, GeticoFect® 3000Plus Reagent has higher transfection efficiency and lower dosage. Like a "money-saving expert", this means that under the condition of achieving the same transfection effect, using GeticoFect® 3000Plus Reagent can reduce reagent consumption, thereby bringing better economic cost-effectiveness to customers.
Taking the 1.5 mL specification product as an example, it is sufficient to complete up to 1500 transfection reactions (in 24-well plates). For scientific research laboratories and biotechnology enterprises, this greatly reduces experimental costs. Especially in large-scale experiments such as drug screening and gene library construction, it can significantly save reagent costs and improve resource utilization efficiency. At the same time, due to its high transfection efficiency and low cytotoxicity, the cost of repeated experiments caused by experimental failure is reduced, further improving economic benefits and making scientific research investment more valuable.
In conclusion, with its advanced nanoparticle technology, outstanding performance, significant advantages, and high cost-effectiveness, GeticoFect® 3000Plus Transfection Reagent is undoubtedly an ideal choice for researchers to conduct cell transfection experiments, and will bring new breakthroughs and developments to biomedical and biotechnology research.
Cat. No. | Specification | Price(￥) |
131201 | 0.75ml | 2600.00 |
131202 | 1.5ml | 4150.00 |
131203 | 15ml | 33200.00 |
1. Q: Compared with GeticoFect 3000, what upgrades have been made to GeticoFect 3000plus in terms of formula and performance?
A: GeticoFect 3000plus has added a targeted delivery enhancement factor to its formula, which can bind more accurately to cell surface receptors and reduce non-specific adsorption. The performance upgrades are mainly reflected in three aspects: first, the transfection efficiency is further improved—for hard-to-transfect cells (such as primary neural cells), the efficiency is 10%-15% higher than that of the 3000 series; second, the toxicity is lower—it adopts a hypotonic liposome structure, and the cell survival rate after transfection is increased by an average of 20%, making it suitable for long-term live cell imaging experiments; third, it is compatible with more types of media, including those with high-concentration serum (>20%) and special additives (such as growth factors), without the need to adjust the culture system.
2. Q: Are there any special requirements for the storage conditions of GeticoFect 3000plus? How long can it be stored after opening?
A: It should be stored in a 4°C refrigerator; freezing or exposure to direct sunlight is strictly prohibited. The shelf life of unopened reagents is 2 years. After opening, it should be sealed and stored at 4°C, and it is recommended to use it up within 3 months. Different from the 3000 series, 3000plus has a shorter stable time at room temperature (20-25°C)—exceeding 1 week may affect its activity. Therefore, if it is accidentally placed at room temperature, it should be returned to 4°C as soon as possible. Before use, it needs to be gently mixed; violent shaking should be avoided to prevent damage to the liposome structure.
3. Q: What are the optimal ratios of GeticoFect 3000plus for transfecting different types of nucleic acids (DNA, siRNA, mRNA)?
A: The ratio needs to be adjusted according to the type of nucleic acid: when transfecting plasmid DNA, the recommended ratio of DNA (μg) to reagent (μL) is 1:1.2-1:2.5; for example, a ratio of 1:1.8 works best for HEK293 cells. When transfecting siRNA/miRNA, due to the stronger binding ability of small molecules, the ratio can be reduced to 1:0.8-1:1.5. When transfecting mRNA, the recommended ratio is 1:1.5-1:3; since mRNA has no nuclear transport step, the amount of reagent needs to be appropriately increased to improve cytoplasmic delivery efficiency. Specific optimization through pre-experiments is required; it is recommended to set 5 gradients (such as 1:0.8, 1:1.2, 1:1.8, 1:2.2, 1:2.8) for each type of nucleic acid to screen the optimal value
4. Q: Can GeticoFect® 3000Plus be used for co-transfection of multiple nucleic acids (e.g., DNA + siRNA, dual plasmids)? If yes, what precautions should be taken?
A: Yes, it supports efficient co-transfection of multiple nucleic acids. For co-transfection scenarios:
• Ratio adjustment: When co-transfecting DNA and siRNA, maintain the total nucleic acid amount within the recommended range for single transfection (e.g., 1 μg total nucleic acid per well in a 24-well plate). The ratio of DNA to siRNA can be adjusted based on experimental needs (common ratios: 1:1, 2:1, 3:1), and the reagent volume should be calculated according to the total nucleic acid mass (following the 1:1.2–1:2.5 μg nucleic acid:μL reagent range).
• Complex formation: Mix all nucleic acids first in serum-free medium, then add the reagent to the mixed nucleic acid solution (do not add reagent to individual nucleic acid solutions separately). Incubate at room temperature for 15–20 minutes to ensure uniform complex formation.
• Cell state: Ensure cells are in the logarithmic growth phase (viability >90%) and maintain a slightly higher confluency (70–80% for adherent cells) than single transfection, as co-transfection may slightly increase cell stress.
5. Q: What should I do if I observe cell aggregation or abnormal morphology after transfection with GeticoFect® 3000Plus?
A: Cell aggregation or abnormal morphology is usually caused by improper operation or environmental factors, with the following solutions:
• Complex concentration: If the reagent-nucleic acid complex concentration is too high, it may cause local cell aggregation. Dilute the complex with more serum-free medium (e.g., double the volume) before adding to cells, and gently swirl the culture plate to ensure even distribution.
• Medium compatibility: Avoid using media containing polyamines, heparin, or high concentrations of divalent cations (e.g., Ca²⁺ > 2 mM) during transfection—these substances may disrupt nanoparticle complex stability and induce cell aggregation. Switch to a standard serum-free medium (e.g., Opti-MEM™) for complex preparation.
• Incubation time: Prolonged incubation of complexes (over 30 minutes) may lead to particle aggregation. Prepare complexes fresh and add them to cells within 15–20 minutes of incubation.
• Cell seeding density: If cells are seeded too densely, post-transfection proliferation may cause aggregation. Adjust the seeding density to ensure 60–70% confluency at the time of transfection.
6. Q: Is GeticoFect® 3000Plus suitable for transfection of primary cells? Which types of primary cells have been validated to work well with this reagent?
A: Yes, it is highly suitable for primary cell transfection, especially when combined with the built-in T3000 enhancer. Validated primary cell types include:
• Primary neural cells: Such as mouse cortical neurons and hippocampal neurons—transfection efficiency can reach 35–45%, and cell survival rate remains >80% 72 hours post-transfection, making it ideal for neural circuit and neurodevelopment research.
• Primary hepatocytes: Including human and rat primary hepatocytes—supports efficient expression of metabolic enzymes (e.g., CYP450) without disrupting liver-specific functions (e.g., albumin secretion).
• Primary fibroblasts: Such as human dermal fibroblasts and mouse embryonic fibroblasts—transfection efficiency >50%, suitable for studies on tissue repair and fibrosis.
• Precautions for primary cells: Use low passage numbers (P0–P2) to maintain cell viability; reduce the reagent-to-nucleic acid ratio by 10–20% compared to cell lines (e.g., 1:1–1:1.8 μg:μL) to minimize cytotoxicity; add 1% BSA to the medium during transfection to enhance cell adhesion and reduce stress.
7. Q: How does GeticoFect® 3000Plus perform in serum-containing medium? Do I need to remove serum before transfection?
A: It exhibits excellent stability and efficiency in serum-containing medium (serum concentration 5–20%) and does not require serum removal before transfection. Key advantages include:
• Serum resistance: The nanoparticle surface is modified with a serum-compatible coating that prevents adsorption of serum proteins (e.g., albumin, immunoglobulins), avoiding complex dissociation or aggregation.
• Operational flexibility: For adherent cells, transfect directly in the existing serum-containing medium; for suspension cells, no need to centrifuge and resuspend in serum-free medium—greatly simplifying the workflow.
• Note: For media with serum concentrations >20% (e.g., some stem cell media), it is recommended to pre-test a small batch first. If transfection efficiency decreases, dilute the medium to 15–20% serum before transfection, or add the complex to the medium and incubate for 4–6 hours before supplementing with additional serum.
8. Q: Can GeticoFect® 3000Plus be used for large-scale transfection (e.g., shake flasks, bioreactors) for recombinant protein production? What are the scaling-up guidelines?
A: Yes, it supports seamless scaling from laboratory-scale (96-well plates) to industrial-scale (200 L bioreactors) for protein production. Scaling-up guidelines:
• Ratio consistency: Maintain the same reagent-to-nucleic acid ratio as small-scale experiments (e.g., 1:1.8 μg DNA:μL reagent). For example, if 1 μg DNA + 1.8 μL reagent is used per well in a 24-well plate (1 mL culture), scale up to 100 mg DNA + 180 mL reagent for 100 L suspension culture.
• Complex preparation: Use a sterile stirred tank (with low-shear impellers) to prepare complexes. First, dilute nucleic acids in 5–10% of the total culture volume (serum-free medium), then add the reagent dropwise while stirring (50–100 rpm), and incubate for 20 minutes.
• Cell density: For suspension cells (e.g., HEK293F), maintain a density of 2–3 × 10⁶ cells/mL at transfection; for adherent cells in bioreactors, use microcarriers and ensure 60–70% confluency on carriers.
• Post-transfection feeding: Add cell culture additives (e.g., glucose, glutamine) 24–48 hours post-transfection to extend the protein expression phase—this can increase yield by 30–50% for secreted proteins.
9. Q: Does GeticoFect® 3000Plus interfere with downstream experiments, such as flow cytometry, Western blotting, or qPCR?
A: No, it has minimal interference with common downstream experiments, thanks to its low residual and biodegradable nanoparticle components. Recommendations for specific experiments:
• Flow cytometry: For intracellular staining, fix cells with 4% paraformaldehyde 48–72 hours post-transfection—residual reagent does not affect antibody binding or fluorescent signal detection. For live cell sorting, wash cells once with PBS before sorting to remove free complexes.
• Western blotting: The reagent does not bind to proteins or interfere with protein separation by SDS-PAGE. Use standard protein extraction protocols (e.g., RIPA buffer) without additional purification steps.
• qPCR: Residual reagent does not inhibit Taq polymerase activity. For nucleic acid extraction, use commercial kits (e.g., Qiagen RNeasy® or DNeasy® Kits)—the reagent’s components are removed during column washing, ensuring accurate qPCR results.
10. Q: What is the recommended time to detect target gene expression or protein production after transfection with GeticoFect® 3000Plus?
A: The detection time varies by nucleic acid type and experimental goal:
• Plasmid DNA (transient expression): For reporter genes (e.g., GFP, luciferase), detect as early as 24 hours post-transfection; for secreted proteins (e.g., antibodies, cytokines), collect supernatant 48–72 hours post-transfection (peak expression usually occurs at 72–96 hours).
• siRNA/miRNA: Gene silencing effects can be detected 24–48 hours post-transfection, with maximum silencing at 48–72 hours. For long-term silencing, repeat transfection every 4–5 days.
• mRNA: Since mRNA is directly translated without nuclear transport, protein expression can be detected as early as 6–12 hours post-transfection, with peak expression at 24–36 hours. mRNA transfection is suitable for short-term protein expression studies (no genomic integration).
For stable cell line generation, select cells with antibiotics (e.g., G418, puromycin) 48 hours post-transfection, and screen for positive clones 2–3 weeks later.
