| Catalog Number | Specification | Price(￥) |
|---|
| 130701 | 0.75ml | 1550.00 |
| 130702 | 1.5ml | 2650.00 |
| 130703 | 15ml | 22000.00 |
In the field of modern biomedical research and biotechnology applications, cell transfection technology is a crucial tool that plays an irreplaceable role in gene function research, drug develop
ment, disease mechanism exploration, and many other aspects. GeticoFect® 2000 Transfection Reagent, as a carefully developed multi-purpose universal transfection reagent, provides researchers with a powerful and reliable transfection solution with its excellent performance and wide applicability.
GeticoFect® 2000 Transfection Reagent demonstrates remarkable applicability in multiple scenarios, providing efficient transfection effects for both adherent and suspension cell lines. Adherent cells, such as common HEK-293 and HeLa cell lines, attach to the surface of culture containers during growth. Transfection of such cells needs to consider the interaction between the reagent and the cell surface, as well as the mechanism of transfection complexes entering the cells. GeticoFect® 2000 Transfection Reagent can effectively bind to the cell membrane of adherent cells, form stable transfection complexes, and then efficiently deliver exogenous nucleic acids into the cells. For suspension cells, such as Jurkat and K562 cell lines, which grow in suspension in the culture medium, transfection is relatively more difficult. Through its unique formula and mechanism of action, GeticoFect® 2000 Transfection Reagent can form a suitable transfection microenvironment around suspension cells, promote nucleic acid uptake, and achieve efficient transfection.
Through a large number of rigorous experimental verifications, GeticoFect® 2000 Reagent has wide application value in the field of gene research. It can be used in gene knockout experiments based on siRNA and shRNA. In gene knockout experiments, siRNA (small interfering RNA) or shRNA (short hairpin RNA) can specifically bind to the mRNA of the target gene, guide the RNA-induced silencing complex (RISC) to cleave it, thereby inhibiting the expression of the target gene. GeticoFect® 2000 Transfection Reagent can efficiently deliver siRNA or shRNA into cells, ensuring that they function in cells to achieve accurate gene knockout effects. At the same time, the reagent can also be used in gene expression research by introducing plasmids or other expression vectors carrying target genes into cells, allowing them to express corresponding proteins in cells, thereby studying gene functions and regulatory mechanisms.
GeticoFect® 2000 Transfection Reagent has shown excellent transfection efficiency in hundreds of cell lines. This is due to its unique chemical composition and optimized formula. Some components in the reagent can form stable complexes with nucleic acids, protect nucleic acids from degradation by intracellular nucleases, and promote the binding and internalization of complexes with cell membranes. On the basis of efficient transfection, it can also achieve a high level of recombinant protein expression. After the exogenous gene is successfully introduced into the cell, the intracellular environment created by GeticoFect® 2000 Transfection Reagent is conducive to the transcription and translation of the gene, enabling a large amount of synthesis and expression of recombinant proteins. This is extremely important for experiments that need to obtain a large amount of recombinant proteins for subsequent research or applications.
In some complex gene research experiments, it is often necessary to introduce siRNA and plasmid DNA into cells simultaneously to achieve precise regulation and functional verification of gene expression. GeticoFect® 2000 Transfection Reagent has excellent co-transfection performance. It can form stable transfection complexes with both siRNA and plasmid DNA, and ensure that both nucleic acids can function effectively after entering the cell. In some studies on gene regulatory networks, researchers can inhibit the expression of a key gene by co-transfecting siRNA, and at the same time introduce a plasmid DNA carrying related regulatory elements to observe changes in gene expression and cell function, thereby gaining an in-depth understanding of the interaction and regulatory mechanism between genes.
With the rapid development of the biotechnology industry, high-throughput experiments have become the key to improving research efficiency and promoting industrial development. GeticoFect® 2000 Transfection Reagent has good stability and repeatability, making it very suitable for high-throughput industrial applications. In large-scale drug screening, gene library construction, and other experiments, a large number of samples need to be transfected simultaneously. The reagent can achieve efficient transfection on high-throughput experimental platforms such as 96-well plates and 384-well plates, and the transfection effect between different wells has high consistency. This not only improves the experimental efficiency but also reduces the experimental cost, providing strong support for industrial production and large-scale research.
GeticoFect® 2000 Transfection Reagent is applicable not only to common cell lines but also to many difficult-to-transfect cell lines. For some special cell lines, such as primary cells and stem cells, due to their physiological characteristics and the particularity of cell membrane structure, traditional transfection reagents often fail to achieve ideal transfection effects. However, through its optimized formula and unique mechanism of action, GeticoFect® 2000 Transfection Reagent can overcome the transfection obstacles of these cell lines and achieve efficient transfection. This makes it possible to study the biological characteristics and functions of these cell lines, expanding the scope of cell research.
The reagent has strong compatibility with transfection samples and can be used for various types of transfection samples such as plasmids, siRNA, and mRNA. Plasmids are common vectors for gene cloning and expression. GeticoFect® 2000 Transfection Reagent can efficiently introduce plasmids into cells to achieve stable expression of exogenous genes. siRNA is used in gene silencing experiments, and the reagent can ensure that siRNA accurately enters cells and functions. mRNA, as an emerging tool for gene therapy and research, can also be efficiently introduced into cells through this reagent to achieve rapid protein expression. This diverse compatibility enables GeticoFect® 2000 Transfection Reagent to meet the needs of different types of experiments, providing researchers with more choices.
The application range of GeticoFect® 2000 Transfection Reagent covers all common cell lines and many difficult-to-transfect cell lines. In different experimental environments, the medium conditions for cell culture are also different. Some experiments need to be carried out in medium containing serum to provide nutrients and growth factors required for cell growth; while others need to be carried out in serum-free medium to avoid interference of serum with experimental results. GeticoFect® 2000 Transfection Reagent can maintain good transfection effects under both medium conditions. In medium containing serum, it can resist the interference of various components in serum and effectively introduce nucleic acids into cells; in serum-free medium, it can also provide a suitable transfection environment for cells to ensure the smooth progress of the transfection process.
In gene silencing applications, the efficient transfection ability of GeticoFect® 2000 Reagent plays an important role. It can efficiently deliver siRNA or shRNA into cells, achieving a high level of gene knockout. By precisely inhibiting the expression of target genes, researchers can conduct in-depth studies on gene functions and related biological processes. In the research of some disease models, gene knockout experiments can determine whether a certain gene is related to the occurrence and development of the disease, providing a theoretical basis for disease diagnosis and treatment. Since GeticoFect® 2000 Reagent can achieve satisfactory gene knockout effects, researchers can obtain accurate and reliable experimental results, promoting the in-depth development of gene research.
GeticoFect® 2000 Transfection Reagent is extremely convenient in operation. It only requires simple preparation: mix the reagent with nucleic acid in a certain proportion, incubate briefly to form transfection complexes, and then directly add them to the cell culture. This simple operation process reduces human errors in the experimental process and improves the repeatability of the experiment. More importantly, it supports a no-medium-change cell transfection system. In traditional transfection experiments, it is often necessary to change the medium after transfection to remove the potential toxicity of the transfection reagent to cells. However, due to its low toxicity and good cell compatibility, GeticoFect® 2000 Transfection Reagent does not require medium change, which not only simplifies the experimental process, saves time and energy but also reduces damage to cells, improving cell survival rate and transfection effect.
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